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            xho造句

            "xho"是什么意思  
            造句與例句手機版
            • Inserted pol ft cdna fragment was recovered from the recombinant vector by xho i and nhe i digesting, and purified
              i和he消化收回在上下游分別有ho11和wb。他性末端的polpcdna片段。
            • According to the mutliclone site of pcdnas plasmid, we have used xho i to cut pcdnas plasmid and make its linearized
              pcdna3和doc一ir的酶切片段25經毛dna連接酶作用形成重組質粒。
            • A 358 bp pol ft cdna fragment framed with introduced restrict sites of xho i ( upstream ) and nhe i ( downstream ) was amplified by the method of rt-pcr, and then cloned into a ta-vector
              rt一pcr擴增得到358hp的pcilpcdna片段,并在其上下游引物中分別引人了h。i和ie內切酶位點。l此片段克隆人ta克隆載體后,經h。
            • Cloning the gene into a pgex expression vector clone the hbrp gene using pcr, restriction digest the pgex-5x-1 vectors and pcr product with bamh i and xho i, recovery the pcr product and pgex-5x-1 vectors
              純化樣品樣品上柱,pbs洗柱后,用洗脫buf企r將蛋白洗脫下來。利用蛋白收集器,收集蛋白,濃縮結果1
            • Reconstruction of antisense pcdnas-doc-1r plasmid after transformation the pmeiss-fl3-doc-1r plasmid into e . coli cells, we have extracted the plasmid and cut doc-1r fragment using xho i to get a 975 bp doc-1r cdna fragment
              pcdna3-doc-1r反義重組質粒的構建將pme18s-fl3-doc-1r質粒轉化后進行質粒提取。用xhoi限制性內切酶從克隆載體上切取975bp的doc-1rcdna片段。
            • The plasmid was tested by the restriction enzyme bamh i and xho i incision, and 1 % agarose gel electrophoresis . the results show that there were two bands at the respected sites about 4.9kb and 750bp respectively . it means that hbrp has been cloned into a pgex-5x-1 expression vector correctly
              2.質粒鑒定pgex一hbrp融合蛋白表達質粒轉化eoh.bi21感受態細胞l)限制性內切酶鑒定提取質粒dna,經bamhfxhol雙酶切鑒定,結果可見,有750bp的插人片段和4.gkb的質粒dna兩條帶,表明所提質粒中含有外源基因hbrp片段,大小及插人方向均正確。
            • After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit . the segment was ligated with vector pmd18-t and then was tranformed into the competent cell of dh5 a . a construction mstnd-pmd18t was generated by inserting the sequence of 254bp into pmd18-t vector and selecting the sense clones . positive clone was identified by three ways : endonuclease digestion, pcr and sequencing . the result showed that the cloned sequence coincides with the designed sequence . this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit . the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ) . after 12-16 hours of culture, several colnes appeared on the plate . some positive clones were selected to extract their plasmid . these plasmids were digested by nco i and xho i and indentified by pcr . a contraction, mstnd-pet28a was generated . the result showed that the cloned sequence coincides with the designed sequence
              f_1長38bp,r_1長36bp,其它片段均40bp長,f_1和r_1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應,然后用其他單鏈片段作為引物,進行pcr擴增,用dna快速純化回收試劑盒回收所得254bppcr產物,與pmd18-t載體連接、轉化dh_5。受體菌感受態細胞,利用藍白斑遺傳學篩選法篩選陽性克隆,提取其質粒,采用nco和xho雙酶切鑒定,獲得了254bp的片段;用pmd18-t載體上的特異引物rv-m和m13-47進行pcr鑒定,獲得300bp的片段。
            • After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit . the segment was ligated with vector pmd18-t and then was tranformed into the competent cell of dh5 a . a construction mstnd-pmd18t was generated by inserting the sequence of 254bp into pmd18-t vector and selecting the sense clones . positive clone was identified by three ways : endonuclease digestion, pcr and sequencing . the result showed that the cloned sequence coincides with the designed sequence . this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit . the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ) . after 12-16 hours of culture, several colnes appeared on the plate . some positive clones were selected to extract their plasmid . these plasmids were digested by nco i and xho i and indentified by pcr . a contraction, mstnd-pet28a was generated . the result showed that the cloned sequence coincides with the designed sequence
              f_1長38bp,r_1長36bp,其它片段均40bp長,f_1和r_1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應,然后用其他單鏈片段作為引物,進行pcr擴增,用dna快速純化回收試劑盒回收所得254bppcr產物,與pmd18-t載體連接、轉化dh_5。受體菌感受態細胞,利用藍白斑遺傳學篩選法篩選陽性克隆,提取其質粒,采用nco和xho雙酶切鑒定,獲得了254bp的片段;用pmd18-t載體上的特異引物rv-m和m13-47進行pcr鑒定,獲得300bp的片段。
            • The ns1 and hns2 gene from aiv ( h9n2 ) isolate were cut from the pmd18-t-nsl vector, the ns1 gene cloned into the sites of bamh i and xho i of prokaryotic expression vector and the hns2 cloned into the sites of ecor i and xho i of prokaryotic expression vector . the recombinant vector was identified by endonuclease
              從含有禽流感病毒分離株非結構蛋白基因的重組質粒pmd18-t-ns1中切取ns1、hns2基因片段,分別將其亞克隆于pgex-6p-1的bamh、xho位點和ecor、xho位點上,然后經單、雙酶切及質粒pcr鑒定,結果表明目的片段基因成功插入表達載體中。
            • The ns1 and hns2 gene from aiv ( h9n2 ) isolate were cut from the pmd18-t-nsl vector, the ns1 gene cloned into the sites of bamh i and xho i of prokaryotic expression vector and the hns2 cloned into the sites of ecor i and xho i of prokaryotic expression vector . the recombinant vector was identified by endonuclease
              從含有禽流感病毒分離株非結構蛋白基因的重組質粒pmd18-t-ns1中切取ns1、hns2基因片段,分別將其亞克隆于pgex-6p-1的bamh、xho位點和ecor、xho位點上,然后經單、雙酶切及質粒pcr鑒定,結果表明目的片段基因成功插入表達載體中。
            • It's difficult to see xho in a sentence. 用xho造句挺難的
            • In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv . the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem-t easy vector . after transforming e . coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr . presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem-3abc . comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent . the pgem-3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii-digested expression vector ptriex-4 neo . lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene . positive clones were selected and induced with lmmol / l isopropyl-d-galactoside ( iptg ), bacteria were detected by sds-page and western blotting after properly treated . the results showed that the 3ab gene expressed successfully in e . coli and 33.5ku fusion protein can be recognized by the positive bovine serum of fmdv . the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
              擴增產物連接到pgem-teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem-3abc和表達載體ptriex-4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex-4neo中,通過酶切鑒定、pcr擴增以及序列分析,發現克隆到ptriex-4neo載體上的片段于3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds-page電泳、westernblotting分析,結果表明,3ab基因在大腸桿菌中成功表達,其表達產物為分子量33.5ku的融合蛋白,并能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。
            • A gus report gene, gained by the method of pcr, was inserted into the pet22b vector between the sites of hindiii and xho i to construct a transitional vector named pet22g then the signal peptide ( pelb leader ) in the pet22g vector was replaced by a containing grb-ast signal peptide fragment to construct expression vector pet22asg which had been transformed into expression host e . coli bl21 ( de3 ) plyss
              先將pcr獲得的gus報告基因插入到經hind和xho雙酶切的pet22b中,構建了中間載體pet22g;然后再將pcr克隆的一含ast信號肽的片段as取代pet22g上的信號肽pelbleader序列,構建了大腸桿菌分泌表達載體pet22asg。
            • Materials and methods in our study, first the e . coli bl21 transformed already are cultivated in smaller scale and then the plasmids dna were extracted by the methods of alkaline lysis . the plasmids dna extraction were processed 37 overnight by the restrictive endo-incisase bam h i and xho i . the incision products were used to check the integrality and variability of the recombinant plasmids dna by 1 % agarose gel elec-trophoresis
              同預想結果基本吻合,大規模培養后純化得到的融合蛋白通過sdspage顯示在52kda處有一條帶,而酶切后產物電泳結果顯示在26kda處分另有兩條蛋白帶,其中一條為gst,另一條為hbrp,基本符合實驗結果;hbrp對tpk活性的抑制呈劑量依賴性。
            如何用xho造句,用xho造句,xho in a sentence, 用xho造句和xho的例句由查查漢語詞典提供,版權所有違者必究。
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